Ultrafiltration characteristics of plasmid DNA
نویسندگان
چکیده
Plasmids are extra-chromosomal circular double-stranded DNA molecules that can be used to develop recombinant cell lines and to deliver therapeutic genetic material. In gene therapy applications, the in-vivo insertion of the genetic vector results in the production of a desired therapeutic bio-molecule. Gene therapy is of interest in the treatment of a wide range of medical conditions including cystic fibrosis, haemophilia, and muscular dystrophy. The plasmids used in clinical trials typically range in size from 5 to 20 kilo-base pairs (kbp), which corresponds to a size on the order of 100 nm.
منابع مشابه
Purification of an Enterobacter aerogenes plasmid DNA using MnCl2 as compaction agent.
Development of an efficient process for purification of DNA plasmids continues to be a technological challenge [1-3]. While earlier applications of DNA plasmids were mostly in cloning, the last few years have seen its increasing applications in gene therapy [1] and preparation of DNA vaccines [4]. A crucial step in the production process is the downstream processing stage which encompasses sepa...
متن کاملانجام آمیختگی مصنوعی در باکتری اشریشیاکلی جهت بررسی انتقال پلاسمید مولد پنی سیلیناز
Conjugation is a major mechanism of DNA transfer among bacteria which takes place in both laboratory and natural environment and brings about new characteristics. Extrachromosomal DNA called plasmid are important elements in this exchange. Resistance plasmid confer resistance to many antibiotics and in this group, penicillinase plasmid is responsible for resistance to penicillins and cephalos...
متن کاملDiffusional Contributions and Electrostatic Exclusion Effects on Transport through Ultrafiltration Membranes
1. Introduction Ultrafiltration is used for protein concentration and buffer exchange in the downstream processing of essentially all recombinant protein products [1]. Although ultrafiltration has traditionally been viewed as a convective pressure-driven process, with the separation determined on the basis of size, solute transport through ultrafiltration membranes is also strongly influenced b...
متن کاملConstruction of a Synthetic Vector for Preparation of a 100 Base Pair DNA Ladder
DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...
متن کاملConstruction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis
Background: With one-third of the world’s population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should s...
متن کامل